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Entry heading AEF1_DROME
Primary accession number P39413
Secondary accession numbers Q0E8C4 Q9VP37
Integrated into Swiss-Prot on February 1, 1995
Sequence was ultimate modified on February 1, 1995 (Sequence version 1)
Annotations be last modified on July 24, 2007 (Entry newspaper 59)
Name and origin of the protein

Protein label Adult enhancer factor 1
Synonym AEF-1
Gene name Name: Aef1
ORFNames: CG5683

From Drosophila melanogaster (Fruit fly) [TaxID: 7227]

Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila.
Protein existence 1: Evidence at protein stratum;

References

[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
TISSUE=Ovary;
PubMed=1508206 [NCBI, ExPASy, EBI, Israel, Japan]
Falb D., Maniatis T.;
"Drosophila transcriptional repressor protein that binds specifically to negative control elements contained by fat body enhancers.";
Mol. Cell. Biol. 12:4093-4103(1992).

[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2... PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).

[3]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.

[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.

Comments

FUNCTION: Transcriptional repressor that binds specifically to podgy body-specific enhancers, namely the adult ADH enhancer (AAE) and the enhancer that controls yolk protein gene expression.
INTERACTION:
Q9VZV5:CG16745; NbExp=1; IntAct=EBI-101725, EBI-498985;
Q9W0P9:CG17181; NbExp=1; IntAct=EBI-101725, EBI-103133;

SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Found in adjectives tissues examined including the ovary and the fat body.
DEVELOPMENTAL STAGE: Higher level are found in third-instar larva and in adults.
SIMILARITY: Contains 4 C2H2-type zinc fingers.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed beneath the Creative Commons Attribution-NoDerivs License.
Cross-references

Sequence databases
EMBL M90755; -; NOT_ANNOTATED_CDS; mRNA. [EMBL / GenBank / DDBJ]
AE014296; AAF51721.1; -; Genomic_DNA. [EMBL / GenBank / DDBJ] [CoDingSequence]
AY060435; AAL25474.1; -; mRNA. [EMBL / GenBank / DDBJ] [CoDingSequence]

PIR A44496; A44496.
UniGene Dm.1036
3D structure databases
HSSP P08046; 1A1H. [HSSP ENTRY / PDB]
ModBase P39413.
Protein-protein interaction databases
DIP DIP:22241N; -.
IntAct P39413; -.
Protein family/group databases
TRANSFAC T01513; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-017708... -.
DMEL-XXX-02:DMEL-XXX-02-017709... -.
DMEL-XXX-02:DMEL-XXX-02-017710... -.
Organism-specific databases
FlyBase FBgn0005694; Aef1.
Gene expression databases
GermOnline CG5683; Drosophila melanogaster.
Ontologies
GO GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.

Family and domain databases
InterPro IPR007087; Znf_C2H2.
Graphical prospect of domain structure.
Pfam PF00096; zf-C2H2; 4.
Pfam graphical view of domain structure.
ProDom PD000003; Znf_C2H2; 3.
[Domain structure / List of seq. sharing at lowest possible 1 domain]
SMART SM00355; ZnF_C2H2; 4.
SMART graphical view of domain structure.
PROSITE PS00028; ZINC_FINGER_C2H2_1; 4.
PS50157; ZINC_FINGER_C2H2_2; 4.
PROSITE graphical estimation of domain structure (profiles).
BLOCKS P39413.
Genome annotation databases
Ensembl CG5683; Drosophila melanogaster. [Contig view]
KEGG dme:Dmel_CG5683; -.
Other
ProtoNet P39413.
UniRef View cluster of proteins with at tiniest 50% / 90% / 100% identity.

Keywords

Complete proteome; DNA-binding; Metal-binding; Nucleus; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.

Features

Feature table viewer Feature aligner
Key From To Length Description FTId
CHAIN 1 308 308 Adult enhancer factor 1. PRO_0000046908
ZN_FING 184 206 23 C2H2-type 1.
ZN_FING 212 234 23 C2H2-type 2.
ZN_FING 240 262 23 C2H2-type 3.
ZN_FING 268 290 23 C2H2-type 4.
COMPBIAS 56 76 21 Gln-rich (OPA repeat).
COMPBIAS 71 76 6 Poly-Gln.
COMPBIAS 105 127 23 Ala-rich.
COMPBIAS 132 135 4 Poly-Pro.

Sequence information

Length: 308 AA [This is down the unprocessed precursor] Molecular weight: 33782 Da [This is the MW of the unprocessed precursor] CRC64: 54F04D90B6FC4250 [This is a checksum on the sequence]

10 20 30 40 50 60
MMHIKSLPHA HAAATAMSSN CDIVIVAAQP QTTIANNNNN ETVTQATHPA HMAAVQQQQQ

70 80 90 100 110 120
QQQQQQQQHH QQQQQQSSGP PSVPPPPTEL PLPFQMHLSG ISAEAHSAAQ AAAMAAAQAA

130 140 150 160 170 180
AAQAAAAEQQ QPPPPTSHLT HLTTHSPTTI HSEHYLANGH SEHPGEGNAA VGVGGAVREP

190 200 210 220 230 240
EKPFHCTVCD RRFRQLSTLT NHVKIHTGEK PYKCNVCDKT FRQSSTLTNH LKIHTGEKPY

250 260 270 280 290 300
NCNFCPKHFR QLSTLANHVK IHTGEKPFEC VICKKQFRQS STLNNHIKIH VMDKVYVPVK

IKTEEDEG

P39413 within FASTA format